全文获取类型
收费全文 | 30796篇 |
免费 | 2760篇 |
国内免费 | 2834篇 |
出版年
2024年 | 25篇 |
2023年 | 307篇 |
2022年 | 452篇 |
2021年 | 1495篇 |
2020年 | 1098篇 |
2019年 | 1333篇 |
2018年 | 1283篇 |
2017年 | 1008篇 |
2016年 | 1350篇 |
2015年 | 1878篇 |
2014年 | 2267篇 |
2013年 | 2493篇 |
2012年 | 2819篇 |
2011年 | 2598篇 |
2010年 | 1707篇 |
2009年 | 1523篇 |
2008年 | 1769篇 |
2007年 | 1607篇 |
2006年 | 1356篇 |
2005年 | 1193篇 |
2004年 | 1015篇 |
2003年 | 988篇 |
2002年 | 912篇 |
2001年 | 582篇 |
2000年 | 524篇 |
1999年 | 497篇 |
1998年 | 305篇 |
1997年 | 259篇 |
1996年 | 260篇 |
1995年 | 226篇 |
1994年 | 222篇 |
1993年 | 146篇 |
1992年 | 180篇 |
1991年 | 142篇 |
1990年 | 137篇 |
1989年 | 98篇 |
1988年 | 68篇 |
1987年 | 58篇 |
1986年 | 54篇 |
1985年 | 44篇 |
1984年 | 27篇 |
1983年 | 20篇 |
1982年 | 26篇 |
1981年 | 12篇 |
1980年 | 6篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1975年 | 4篇 |
1950年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 171 毫秒
91.
92.
The availability of DNA structural probes that can be applied to living cells is essential for the analysis of biological functions of unusual DNA structures adopted in vivo. We have developed a chemical probe assay to detect and quantitate left-handed Z-DNA structures in recombinant plasmids in growing E. coli cells. Potassium permanganate selectively reacts with B-Z or Z-Z junction regions in supercoiled plasmids harbored in the cells. Restriction enzyme recognition sites located at these junctions are not cleaved by the corresponding endonuclease after modification with KMnO4. This inhibition of cleavage allows the determination of the relative amounts of B- and Z-forms of the cloned inserts inside the cell. We have successfully applied this method to monitor the extent of Z-DNA formation in E. coli as a function of the growth phase and mutated topoisomerase or gyrase activities. The assay can in principle be used for any unusual DNA structure that contains a restriction recognition site inside or near the structural alteration. It can be a useful tool to analyze in vivo correlations between DNA structure and gene regulatory events. 相似文献
93.
94.
Ailiang Jiang 《International journal of biometeorology》1988,32(4):280-282
According to the author's and his collaborators' investigations, the climate influences the growth of rubber trees (Hevea brasiliensis) in Xishuangbanna, the southern part of Yunnan Province, China, in at least four aspects: (1) The yield of latex per tapping and the final yield of dry rubber per tree per year or per unit area per year; (2) the growth rate, as expressed by increment of girth in cm; (3) the survival during the over-wintering period; (4) the initiation or suppression of certain diseases; In this paper the author would like to describe the influence of climatic elements on yield of latex and on survival during the over-wintering period. As for the other two aspects, only general comments are given. 相似文献
95.
96.
从自然感染的意大利麻痹病蜂(APis mellifera)头部,经二次差速离心与蔗糖梯度离心获得纯化的慢性蜜蜂麻痹病病毒(CBPV)。纯化的CBPV制备物感染正常蜜蜂,4天后出现典型的麻痹症状,接着死亡,平均死亡率分别为95%与100%。SDS-聚丙烯酰胺凝胶电泳分析,二次差速离心初步纯化的病毒制备物含有多条蛋白带,而蔗糖密度梯度纯化的病毒制备物仅含有单一的多肽带。5%、7.5%与10%SDS-聚丙烯酰胺凝胶电泳分析,均检测出一种病毒蛋白质,分子量大约为24,200道尔顿,而且不同凝胶浓度检测的蛋白质分子量相近。慢性蜜蜂麻痹病病毒核酸也用SDS-聚丙烯酰胺凝胶电泳分析,结果表明,凝胶中有5条带,对核酸酶敏感,证明该病毒含有5个单股RNA组分。对慢性蜜蜂麻痹病病毒的基因组结构进行了讨论。 相似文献
97.
98.
Mu-Chin Tzeng Ming Jhy Hseu Jun Hai Yang Richard John Guillory 《The protein journal》1986,5(3):221-228
Snake presynaptic toxins such as crotoxin, β-bungarotoxin and taipoxin block neuromuscular transmission through inhibiting the release of acetylcholine by their phospholipase A2 activities. On the other hand, many other phospholipase A2s show little neurotoxicity. It is likely that the difference lies in whether high affinity binding to nerve cell membranes exists or not. To test this idea, crotoxin, β-bungarotoxin and taipoxin were first radioactively labeled with Na(125I) without loss of their neurotoxicity. Using the radioactive toxins we have found that each of the three showed specific binding to synaptosomal membranes from guinea pig brain. In contrast, we could not detect specific binding of a non-neurotoxic pancreatic phospholipase A2. Crotoxin and taipoxin, but not β-bungarotoxin, also bound specifically to membrane preparation from other tissues. The binding of each toxin was not greatly affected by the other two toxins. The photoaffinity labeling technique has been used to obtain further information about the components which bind crotoxin. For this purpose, (125I) crotoxin was derivatized with N-hydroxysuccinimidyl-4-azidobenzoate. Autoradiographic analysis of the membranes following photoirradiation in the presence of the modified crotoxin revealed that an 85K dalton component was preferentially covalently conjugated with the crotoxin analogue in a specific manner. 相似文献
99.
Individual 14C-labelled amino acids are rapidly removed from dilute solution in artificial sea water (0.2 mol 1–1) by suspensions of Meliosira medocris. The rate of disappearance of radioactivity corresponds closely to removal of primary amines as determined by measurement of the rate of decrease of fluorescamine-positive material. Net removal of naturally occurring free amino acids from the sea water habitat from which the alga was isolated is demonstrated using high performance liquid chromatography. Removal of amino acids from natural sources makes a significant contribution to the carbon requirements of the alga as well as supplying significant amounts of amino nitrogen. 相似文献
100.